Stem Cell Processing Technician

Job Description: Cell Processing Technician — PRP & Bone Marrow–Derived Cell FractionsOrganisation:ALIV Regenerative Wellness Location:Mumbai |Type:Full-time |Mode:On-site Reports to:Lab/Clinical Operations Lead1 Month TRAINING in PUNEAbout ALIVALIV is a compliant regenerative wellness clinic focused onAutologous Cell Therapy ,PRP/PRFM , andtargeted IV therapies , delivered with strict ethics and SOPs.Role SummaryHands-on technician toprocess PRP/PRFMandbone marrow–derived autologous cell fractions (minimally manipulated)with impeccable aseptic technique, documentation, and turnaround time. You’ll partner with clinicians to ensure safe, consistent, and reproducible outputs for patient procedures.Key ResponsibilitiesSample Handling & Chain of CustodyReceive, label, and track blood and bone-marrow aspirate; maintain time/temperature controls and transport logs. Verify consent, requisitions, and kits; ensure traceability from collection to reinfusion.PRP / PRFM ProcessingPerform anticoagulation, centrifugation per protocol (soft/hard spins), plasma separation, platelet resuspension, PRFM activation. Record volumes, spin parameters, platelet yield/concentration, and lot numbers of disposables.Bone Marrow–Derived Cell Fraction ProcessingProcess aspirate using validatedclosed/open systems(e.g., RBC depletion, density separation). Isolate mononuclear/cell fractions as per SOP; prepare final product for clinical use.Asepsis, QC & ReleaseWork underClass II biosafety cabinet/laminar flow ; follow sterile technique and PPE. Perform in-process QC:TNC/platelet counts, viability (e.g., Trypan Blue), recovery %, hemolysis check , appearance. Prepare batch records andCertificate of Processing/Releasefor clinician sign-off.Equipment & FacilityOperate and maintaincentrifuges (fixed/swing), pipettes, cell counters/hemocytometer, vortex, incubators (if applicable) . Daily/weeklycalibration, IQ/OQ/PQ logs , maintenance tickets, and environmental hygiene records.Compliance & SafetyAdhere toDCGI/ICMR guidance , clinic SOPs, biosafety, biomedical waste rules, and incident/AER reporting. Participate in audits, CAPA, and periodic validation runs.Inventory & CoordinationManage stock ofkits, anticoagulants, reagents, disposables ; raise purchase requests proactively. Coordinate OR/clinic schedules for timely processing and delivery.QualificationsB.Sc./M.Sc.in Biotechnology/Microbiology/Biomedical/Allied Healthor DMLT/BMLT . 2–5 yearsin a cell/hematology/transfusion, IVF, orthobiologics, or day-care lab (PRP/BM processing preferred). Strong grasp of aseptic technique, centrifugation theory, and basic hematology counts. Comfortable withEMR/CRM/LIS , Google Workspace, and documentation discipline. Languages:English + Hindi/Marathi (or local) .Skills & TraitsMeticulous, steady hands, andSOP-firstmindset. Data-accurate; converts protocols into repeatable outputs. Calm under time pressure; clear communication with clinicians and nursing. Integrity, patient privacy, and teamwork.KPIsYield & viabilitywithin spec;turnaround timeadherence. Documentation accuracy(batch records, logs) and SOP compliance. Zero contamination/incident rate ; successful audits. Stockouts avoided; equipment uptime.90-Day Outcomes30 days:Trained and certified on ALIV SOPs; independent PRP runs with complete records. 60 days:Independent bone-marrow fractionation; QC dashboards live. 90 days:≥95% runs within yield/viability specs; audit-ready documentation; proposes at least2process improvements.

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